海外で話題のDE-T1とは
DE-T1の卵巣顆粒膜細胞への影響
DE-T1が卵巣顆粒膜細胞の増殖に与える影響
DE-T1は、卵巣顆粒膜細胞の増殖能力を向上させることが示されています。DE-T1濃度が上昇すると、細胞の生存能力も徐々に上昇します[5]。
DE-T1が卵巣顆粒膜細胞のホルモン受容体に与える影響
以前の研究で、DE-T1は卵巣顆粒膜細胞の表面にあるFSH受容体の活性を増加させることが確認されています[2][3]。DE-T1添加により、FSHR、LHR、IGF-1R、およびCYP19A1のmRNA発現がアップレギュレーションされました[5]。
DE-T1の妊娠率への影響
DE-T1が体外受精治療における胚の成長に与える影響
DE-T1は、体外受精治療を受ける女性の胚盤胞獲得率を向上させることが示されています[6(]。
DE-T1が体外受精治療における生児出産率に与える影響
DE-T1添加群の生児出産率は、非添加群よりも有意に高く（57.53％対40.0％、P=0.045）、DE-T1は体外受精胚移植治療（IVF-ET）を受ける女性の生児出産率を向上させる可能性があるとされています[7]。
結論
DE-T1は、卵巣顆粒膜細胞の増殖能力とホルモン受容体の発現を増加させ、体外受精治療における胚盤胞獲得率および生児出産率を向上させる可能性があります。これにより、DE-T1は不妊治療の一環として有望な補助治療法となることが期待されます。
よくある質問
Q1: DE-T1はどのように卵巣顆粒膜細胞に作用しますか？
A1: DE-T1は、卵巣顆粒膜細胞の増殖能力を向上させることが示されており、また、FSH受容体の活性を増加させることが確認されています[2][3][5]。
Q2: DE-T1は体外受精治療にどのように影響しますか？
A2: DE-T1は、体外受精治療における胚盤胞獲得率および生児出産率を向上させることが示されています
Q3: DE-T1はどのような濃度で顆粒膜細胞に効果がありますか？
A3: 研究によれば、DE-T1の濃度が上昇するにつれて顆粒膜細胞の生存率が徐々に増加し、特に2.5%のDE-T1濃度でFSHR、LHR、IGF-1R、およびCYP19A1のmRNA発現が最も高まることがわかりました[5]。
Q4: DE-T1の副作用はありますか？
A4: 現時点での研究では、DE-T1の副作用については明確に報告されていません。ただし、個々の研究や臨床試験において詳細に検討される必要があります。
Q5: DE-T1はすべての不妊治療に適用できますか？
A5: DE-T1は卵巣顆粒膜細胞の増殖能力とホルモン受容体の発現を増加させる効果があるため、特に体外受精治療において有望な補助治療法となる可能性があります。しかし、すべての不妊治療に適用できるかどうかは、個々の状況や原因により異なります。医師と相談して最適な治療法を選択してください。
参考資料
1 “Jul 20, 2018 · Effect of dandelion extract T-1 on in vitro proliferation of human granulosa cells. Effect of different concentrations of dandelion extract T-1 on the viability of granulosa cells cultured in vitro. After GCs were cultured with different concentrations of DE-T1 for 48 h, the CCK-8 assay was used to test cell viability.”
URL: https://journals.lww.com/cmj/Fulltext/2018/07200/Effect_of_Dandelion_Extracts_on_the_Proliferation.9.aspx
2 “Jul 20, 2018 · Our previous studies have con rmed that DE-T1 could increase the activity of FSH receptors on the surface of ovarian granulosa cells 7. In this study, DE-T1 supplementation signi cantly …”
URL: https://www.researchgate.net/publication/326167580_Effect_of_Dandelion_Extracts_on_the_Proliferation_of_Ovarian_Granulosa_Cells_and_Expression_of_Hormone_Receptors
3 “Our previous studies have confirmed that DE-T1 could increase the activity of FSH receptors on the surface of ovarian granulosa cells 7. In this study, DE-T1 supplementation significantly increased the blastocyst obtained rate in women undergoing IVF treatment.”
URL: https://www.researchsquare.com/article/rs-900217/v1
4 “May 24, 2021 · The National Cancer Database’s analysis of 2680 women with GCT showed that AC had no effect on 5-year survival rate. 41 However, Park et al concluded that receiving six cycles of BEP regimen after surgery is significantly associated with improved survival; among the six advanced patients who received six cycles of BEP, none of them relapsed, whereas 71.4% (5/7) relapsed and 42.9% (3/7) died of …”
URL: https://obgyn.onlinelibrary.wiley.com/doi/full/10.1111/aogs.14189
5 “Jul 20, 2018 · Results: The cell viability gradually increased with the progressive increase in the DE-T1 concentration. Compared with the control group (without DE-T1), the mRNA expressions of FSHR, LHR, IGF-1R, and CYP19A1 were upregulated after the addition of DE-T1, especially in the 2.5% DE-T1 group (P \< 0.01).”
URL: https://pubmed.ncbi.nlm.nih.gov/29998889/
6 “Sep 13, 2021 · Conclusion DE-T1 supplementation might be an influence on improving the blastocyst obtained rate and live birth rates in women receiving IVF-ET treatment. DE-T1 supplementation might be of benefit …”
URL: https://www.researchgate.net/publication/355012848_DE-T1_On_The_Blastocyst_Obtained_Rate_and_Live_Births_Rates_in_Women_Receiving_IVF-ET_Treatment
7 “The live birth rate of the supplementation group was significantly higher than that of the non-supplementation group (57.53% vs. 40.0%, P=0.045). Conclusion: DE-T1 supplementation might be an influence on improving the blastocyst obtained rate and live birth rates in women receiving IVF-ET treatment. DE-T1 supplementation might be of benefit to …”
URL: https://www.fortunejournals.com/articles/det1-on-the-blastocyst-obtained-rate-and-live-births-rates-in-women-receiving-ivfet-treatment.html
8 “Aug 6, 2018 · Apoptosis of granulosa cells seems to have a negative effect on conception and pregnancy rates in IFV programs. Thus, in this review we present a brief outline of clinical correlation of apoptosis in human granulosa cells and cumulus cells, and its influence upon oocyte quality and IFV outcome. Taken together, understanding the influence of …”
URL: https://onlinelibrary.wiley.com/doi/10.1002/cbin.11036
9 “Sep 1, 2018 · Aim: In this study, we have therefore analysed the influence of vitrification on the survival rates of granulosa cells, the impact of DMSO or ethylenglycol containing vitrification protocols and …”
URL: https://www.researchgate.net/publication/327865524_Impact_of_vitrification_on_granulosa_cell_survival_and_gene_expression
10 “The live birth rate per embryo transfer was 43.4% with an implantation rate per embryo of 17.6%. Of the untransferred embryos, 68% developed to the blastocyst stage and were cryopreserved. We conclude that the simple system of autologous granulosa-lutein cell co-culture improves embryo development, implantation and subsequent pregnancy rates …”
URL: https://pubmed.ncbi.nlm.nih.gov/7769072/
11 “Concerning transfection performed using chemical reagents, the potential effect of electroporation by itself (without transfection of nucleic acids) on a specific granulosa cell function, the …”
URL: https://www.researchgate.net/figure/Transfection-rate-of-granulosa-cells-after-electroporation-with-a-fluorescent-MO-using_fig3_276074809
12 “Apr 27, 2022 · These follicles in PCOS present with a relative lack of granulosa cells and/or degenerating granulosa cells (GCs) (4, 5), indicating abonormal GC proliferation and/or apoptosis. Accumulating evidence indicates that granulosa cells are essential in determining follicular fate by providing the oocyte with nutrients and growth regulators .”
URL: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9094420/